hcc cell lines smmc7721  (Procell Inc)

Journal: Journal of Cancer

Article Title: LINC01535 promotes hepatocellular carcinoma proliferation and metastasis by regulating the miR-214-3p/VASP axis

doi: 10.7150/jca.91756

Figure Lengend Snippet: LINC01535 knockdown suppressed HCC cell proliferation, migration and invasion. A. The expression level of LINC01535 was detected by qRT-PCR after shRNA transfection. B. Growth curves based on the CCK-8 assay results of Focus and HCCLM3 cells with LINC01535 silencing. C. The number of colonies was reduced after LINC01535 downregulation in HCCLM3 and Focus cells. D. A wound healing assay was conducted to measure the migration ability of HCCLM3 and Focus cells after different treatments. E. Transwell assays suggested that LINC01535 knockdown inhibited HCC cell migration and invasion. (* P<0.05, ** P<0.01, *** P<0.001).

Article Snippet: HCC cell lines (HCCLM3, Hep3B, Focus, HepG2 and SMMC7721) and immortalized human hepatocyte L02 cells were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Knockdown, Migration, Expressing, Quantitative RT-PCR, shRNA, Transfection, CCK-8 Assay, Wound Healing Assay

Journal: Journal of Cancer

Article Title: LINC01535 promotes hepatocellular carcinoma proliferation and metastasis by regulating the miR-214-3p/VASP axis

doi: 10.7150/jca.91756

Figure Lengend Snippet: LINC01535 overexpression promoted HCC cell proliferation, migration and invasion. A. Assessment of the transfection efficiency of LINC01535 overexpression lentivirus. B-C. CCK-8 and colony formation assays were used to determine the growth and colony formation capacities of YY8103 and Hep3B cells transfected with either NC or LINC01535-ov. D-E. The wound healing assay and Transwell assays showed that HCC cells transfected with LINC01535 overexpression lentivirus exhibited stronger migration and invasion ability. (* P<0.05, ** P<0.01, *** P<0.001).

Article Snippet: HCC cell lines (HCCLM3, Hep3B, Focus, HepG2 and SMMC7721) and immortalized human hepatocyte L02 cells were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Over Expression, Migration, Transfection, CCK-8 Assay, Wound Healing Assay

Journal: Journal of Cancer

Article Title: LINC01535 promotes hepatocellular carcinoma proliferation and metastasis by regulating the miR-214-3p/VASP axis

doi: 10.7150/jca.91756

Figure Lengend Snippet: LINC01535 increased HCC cell growth and metastasis in vivo. A. Images of subcutaneous tumor tissues formed by HCCLM3 cells transfected with LINC01535-sh2 or sh-NC. The tumor volume and weight were recorded and analyzed. B. Images of subcutaneous tumor tissues formed by YY8103 cells transfected with LINC01535-ov or NC. The tumor volume and weight were recorded and analyzed. C. HE staining of lung tissue in different treatment groups (right, 20x) and analysis of the incidence of lung metastasis (left). (*** P<0.001).

Article Snippet: HCC cell lines (HCCLM3, Hep3B, Focus, HepG2 and SMMC7721) and immortalized human hepatocyte L02 cells were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: In Vivo, Transfection, Staining

Journal: Journal of Cancer

Article Title: LINC01535 promotes hepatocellular carcinoma proliferation and metastasis by regulating the miR-214-3p/VASP axis

doi: 10.7150/jca.91756

Figure Lengend Snippet: VASP partially reversed the function of miRNA-214-3p and LINC01535 regulated PI3K/Akt signaling and EMT via VASP. A. The expression of VASP in HCCLM3 and YY8103 cells subjected to different treatments. B-C. The growth and colony formation capacities of the indicated groups were detected through CCK-8 and colony formation assays. D-E. Wound healing and Transwell assays demonstrated that miR-214-3p impaired the migration and invasion of HCC cells, while VASP reversed the effects caused by miR-214-3p. F. Knocking down LINC01535 upregulated E-cadherin and downregulated VASP, p-AKT, p-mTOR and Vimentin proteins in HCCLM3 cells, while the opposite changes were observed in YY8103 cells overexpressing LINC01535. (* P<0.05, ** P<0.01, *** P<0.001).

Article Snippet: HCC cell lines (HCCLM3, Hep3B, Focus, HepG2 and SMMC7721) and immortalized human hepatocyte L02 cells were obtained from the China Center for Type Culture Collection (Wuhan, China).

Techniques: Expressing, CCK-8 Assay, Migration

Journal: Cell Death & Disease

Article Title: Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway

doi: 10.1038/s41419-018-0902-5

Figure Lengend Snippet: a Polyphyllin I exhibited the most obvious inhibitory effect on VM formation. b Structure of PPI. c Effects of PPI on the viability of SMMC7721, PLC, HepG2, Hep3B, and Bel7402 cells. PPI reduced the survival rate of HCC cells at relatively high concentrations and had slight influence on cell survival below 1 μM. d Cell cycle analysis of PPI in HCC cell lines. 0.1 μM PPI did not influence the cycle of HCC cells. e , f PPI inhibited the migration and invasion of HCC cells. g PPI decreased the number of pipe-like structures in SMMC7721 cells on Matrigel. h Time-lapse images of SMMC7721. PPI inhibited the pipe-like structure formation in SMMC7721 cells ( n = 3)

Article Snippet: HCC cell lines including SMMC7721, PLC, HepG2, Hep3B, and Bel7402 were purchased from Keygene BioTECH (Nanjing, China) and validated through a short tandem repeat-based method.

Techniques: Cell Cycle Assay, Migration